Crispr-Cas Methods

Volume two of this series collects refreshed and newly added laboratory techniques for working with CRISPR-Cas systems. Its protocols cover prime editing, base editing, and multiplex editing, along with editing carried out in cell-free extracts. The book also walks through computational checks of guide RNA secondary structure and the use of CRISPR for diagnostic purposes.

The manual is intended as a hands-on reference for researchers who want a broad picture of CRISPR-Cas approaches and how they are applied in practice. Its examples span the genetic modification of crop plants, cultured cell lines, nematodes, and microorganisms.

One chapter, on CRISPR/Cas9-mediated gene editing in human induced pluripotent stem cells, is freely available online under a Creative Commons Attribution 4.0 International License through link.springer.com.