Gene expression profiling

The transcription of messenger RNA from a DNA template is a key process in a wide variety of biological systems. In this book, leaders in gene expression methodology and bioinformatics data analysis share their best methods for measuring RNA levels in cells and tissues. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.