Genome Editing

This practical guide is written for investigators who want to learn how to use CRISPR-Cas9-based technologies, with a particular focus on cardiovascular research and clinical applications. It moves from the basics of genome editing through design considerations to the assessment of results and the ways the technology is applied.

The aim is to let readers get started and build a complete workflow, from the first stage of a project to its completion. Worked examples come from real experiments, and the book also covers troubleshooting and pitfalls to avoid. It is intended as a reference for researchers in both cardiovascular and wider biomedical research.