Gradient HPLC for Practitioners

Analytical chemists who run liquid chromatography will find this hands-on guide focused on gradient methods, where the solvent composition changes during a run. It starts with the basic principles behind gradient separations and then moves to the situations practitioners meet most often. These include coupling LC with mass spectrometry, separating biological molecules, and handling ionic compounds. Throughout, the book gives practical detail intended to help readers apply gradient techniques with confidence and efficiency.