HLA Typing

Researchers working on human leukocyte antigen genotyping will find this volume useful as the field changes quickly. It covers laboratory approaches, including high-throughput platforms, bench protocols, and the use of microarray results. It also examines computational methods that infer HLA alleles and haplotype frequencies from DNA and RNA sequencing data.

The book follows the format of the Methods in Molecular Biology series. Each chapter opens with background on its topic, then lists the materials and reagents needed. Step-by-step protocols are written so they can be repeated in other labs, and each one is followed by advice on troubleshooting and on steering around common errors.

The chapters are aimed at practical use, making the book suited to any researcher who wants to learn the techniques used in this developing area.