Light Sheet Fluorescence Microscopy

This volume explains light sheet fluorescence microscopy, an imaging method that has changed how researchers can carry out experiments and capture data that was previously out of reach. Contributors from physics, biology and computer science cover the foundations of the technique, the instruments used to build and run it, its applications, how samples are prepared, and how the resulting data is analysed. The chapters combine established methods with newer approaches and practical advice drawn from hands-on work.

Several chapters come from leading instrument makers, including Zeiss, Leica, Lavision Biotech, Phase View and ASI, and describe their current systems and recent developments. The book also walks through building a do-it-yourself light sheet microscope, a route to making the method affordable for any laboratory. Hardware and software options are described in detail to help readers set up the technique in their own lab.

Written as an accessible introduction for newcomers and a source of tips for experienced users, the book is aimed at cell biologists, biophysicists, developmental biologists and neurobiologists who want an overview of the latest applications.