Protein sequencing protocols

This compilation gathers up-to-date laboratory methods for determining the amino acid order of proteins. Chapters explain how to run automated sequencers and mass spectrometers, and how to locate where proteins carry chemical modifications. Readers also find procedures for cutting proteins into peptides and mapping them, and for measuring amino acids after they have been chemically altered before or after separation. The volume also covers preparing proteins and peptides in amounts as small as a microgram or less, and sequencing peptides from either end using both established and newer approaches.