Protoplast Technology

Scientists who are developing or applying protoplast methods today contributed the laboratory procedures gathered in this collection. The opening section covers the core techniques and what they make possible, including how protoplasts are isolated, how genetic material is introduced into them through transfection, and how they are regenerated. Several chapters show how protoplasts support genome editing and gene function studies in major crop and model plant species.

The later chapters turn to more advanced areas: automated protoplast handling, large-scale functional genomics, and synthetic biology. Each chapter begins with an introduction to its topic and then lists the materials and reagents required. Step-by-step procedures follow, written so they can be repeated in the lab, with troubleshooting advice and warnings about common pitfalls included throughout.

The volume belongs to Springer's Methods in Molecular Biology series and is intended for plant researchers who want to adopt or refine protoplast-based work in plant genomics. The contributors hope it will encourage the next generation of scientists to improve these protocols and speed up progress in the field.